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GENE - CHEMICAL INTERACTIONS REPORT

CRRD ID: 736871
Species: Homo sapiens
CRRD Object: Gene
Symbol: DHFR
Name: dihydrofolate reductase
Acc ID: CHEBI:44185
Term: methotrexate
Definition: A pteridine that has formula C20H22N8O5.
Chemical ID: MESH:D008727
Note: Use of the qualifier "multiple interactions" designates that the annotated interaction is comprised of a complex set of reactions and/or regulatory events, possibly involving additional chemicals and/or gene products.
QualifierEvidenceWithReferenceSourceNotesOriginal Reference(s)
affects response to substanceEXP 6480464CTDDHFR 3' UTR SNP affects the susceptibility to Methotrexate

PMID:17686970
decreases expressionEXP 6480464CTDMethotrexate results in decreased expression of DHFR mRNA

PMID:23897011, PMID:24657277
decreases response to substanceEXP 6480464CTDDHFR mRNA results in decreased susceptibility to Methotrexate, DHFR protein results in decreased susceptibility to Methotrexate

PMID:14679136, PMID:18694510, PMID:19732436, PMID:28188287
increases expressionEXP 6480464CTDMethotrexate results in increased expression of DHFR mRNA, Methotrexate results in increased expression of DHFR protein

PMID:19570950, PMID:22484375, PMID:23799623, PMID:23838799, PMID:24736981
multiple interactionsEXP 6480464CTD[[MIR125A mRNA binds to DHFR 3' UTR] which results in decreased expression of DHFR protein] which results in increased susceptibility to Methotrexate, [[MIR25 mRNA binds to DHFR 3' UTR] which results in decreased expression of DHFR protein] which results in increased susceptibility to Methotrexate, [ADAR protein results in increased expression of DHFR protein] which results in decreased susceptibility to Methotrexate, [DHFR 3' UTR SNP affects the expression of DHFR protein] which affects the susceptibility to Methotrexate, [DHFR 3' UTR SNP affects the stability of DHFR mRNA] which affects the susceptibility to Methotrexate, [Folic Acid deficiency co-treated with Methotrexate] results in decreased expression of DHFR mRNA

PMID:17686970, PMID:24657277, PMID:28188287
decreases activityISORGD:104716480464CTDMethotrexate results in decreased activity of DHFR protein

PMID:22806879
decreases expressionISORGD:104716480464CTDMethotrexate results in decreased expression of DHFR mRNA, Methotrexate results in decreased expression of DHFR protein

PMID:30502384
decreases expressionISORGD:25006480464CTDMethotrexate results in decreased expression of DHFR mRNA, Methotrexate results in decreased expression of DHFR protein

PMID:30502384
decreases response to substanceISORGD:104716480464CTDDHFR mRNA results in decreased susceptibility to Methotrexate, DHFR protein results in decreased susceptibility to Methotrexate

PMID:16918715
increases expressionISORGD:104716480464CTDMethotrexate results in increased expression of DHFR protein

PMID:22484375
multiple interactionsISORGD:104716480464CTDMethotrexate promotes the reaction [E2F1 protein binds to DHFR promoter]

PMID:22484375
multiple interactionsISORGD:25006480464CTDDHFR protein inhibits the reaction [Methotrexate results in decreased expression of CASP3 protein], DHFR protein inhibits the reaction [Methotrexate results in decreased expression of GFAP mRNA], DHFR protein inhibits the reaction [Methotrexate results in decreased expression of MTHFR mRNA], DHFR protein inhibits the reaction [Methotrexate results in decreased expression of MTRR mRNA], DHFR protein inhibits the reaction [Methotrexate results in increased cleavage of PARP protein], Folic Acid inhibits the reaction [Methotrexate results in decreased expression of DHFR mRNA]

PMID:30502384
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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.