Huntingtin associated protein 1 regulates trafficking of the amyloid precursor protein and modulates amyloid beta levels in neurons.

Authors: Yang, GZ  Yang, M  Lim, Y  Lu, JJ  Wang, TH  Qi, JG  Zhong, JH  Zhou, XF 
Citation: Yang GZ, etal., J Neurochem. 2012 Sep;122(5):1010-22. doi: 10.1111/j.1471-4159.2012.07845.x.
Pubmed: (View Article at PubMed) PMID:22731248
DOI: Full-text: DOI:10.1111/j.1471-4159.2012.07845.x

Amyloid precursor protein (APP) is involved in the pathogenesis of Alzheimer's disease. It is axonally transported, endocytosed and sorted to different cellular compartments where amyloid beta (Abeta) is produced. However, the mechanism of APP trafficking remains unclear. We present evidence that huntingtin associated protein 1 (HAP1) may reduce Abeta production by regulating APP trafficking to the non-amyloidogenic pathway. HAP1 and APP are highly colocalized in a number of brain regions, with similar distribution patterns in both mouse and human brains. They are associated with each other, the interacting site is the 371-599 of HAP1. APP is more retained in cis-Golgi, trans-Golgi complex, early endosome and ER-Golgi intermediate compartment in HAP1-/- neurons. HAP1 deletion significantly alters APP endocytosis and reduces the re-insertion of APP into the cytoplasmic membrane. Amyloid precursor protein-YFP(APP-YFP) vesicles in HAP1-/- neurons reveal a decreased trafficking rate and an increased number of motionless vesicles. Knock-down of HAP1 protein in cultured cortical neurons of Alzheimer's disease mouse model increases Abeta levels. Our data suggest that HAP1 regulates APP subcellular trafficking to the non-amyloidogenic pathway and may negatively regulate Abeta production in neurons.


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CRRD Object Information
CRRD ID: 10047200
Created: 2015-07-11
Species: All species
Last Modified: 2015-07-11
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.