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Intraneuronal accumulation of misfolded tau protein induces overexpression of Hsp27 in activated astrocytes.

Authors: Filipcik, P  Cente, M  Zilka, N  Smolek, T  Hanes, J  Kucerak, J  Opattova, A  Kovacech, B  Novak, M 
Citation: Filipcik P, etal., Biochim Biophys Acta. 2015 Jul;1852(7):1219-29. doi: 10.1016/j.bbadis.2015.03.003. Epub 2015 Mar 12.
Pubmed: (View Article at PubMed) PMID:25772164
DOI: Full-text: DOI:10.1016/j.bbadis.2015.03.003

Accumulation of misfolded forms of microtubule associated, neuronal protein tau causes neurofibrillary degeneration typical of Alzheimer's disease and other tauopathies. This process is accompanied by elevated cellular stress and concomitant deregulation of heat-shock proteins. We used a transgenic rat model of tauopathy to study involvement of heat shock protein 27 (Hsp27) in the process of neurofibrillary degeneration, its cell type specific expression and correlation with the amount of insoluble tau protein aggregates. The expression of Hsp27-mRNA is more than doubled and levels of Hsp27 protein tripled in aged transgenic animals with tau pathology. The data revealed a strong positive and highly significant correlation between Hsp27-mRNA and amount of sarkosyl insoluble tau. Interestingly, intracellular accumulation of insoluble misfolded tau protein in neurons was associated with overexpression of Hsp27 almost exclusively in reactive astrocytes, not in neurons. The topological dissociation of neuronally expressed pathological tau and the induction of astrocytic Hsp27, GFAP, and Vimentin along with up-regulation of microglia specific markers such as CD18, CD68 and C3 point to cooperation of astrocytes, microglia and neurons in response to intra-neuronal accumulation of insoluble tau. Our data suggest that over expression of Hsp27 represents a part of microglia-mediated astrocytic response mechanism in the process of neurofibrillary degeneration, which is not necessarily associated with neuroprotection and which in contrary may accelerate neurodegeneration in late stage of the disease. This phenomenon should be considered during development of disease modifying strategies for treatment of tauopathies and AD via regulation of activity of Hsp27.

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CRRD Object Information
CRRD ID: 10402580
Created: 2015-10-27
Species: All species
Last Modified: 2015-10-27
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.