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Identification of mimotopes of platelet autoantigens associated with autoimmune thrombocytopenic purpura.

Authors: Gevorkian, G  Manoutcharian, K  Govezensky, T  Cano, JA  Dominguez, V  Santamaria, H  Larralde, C 
Citation: Gevorkian G, etal., J Autoimmun. 2000 Aug;15(1):33-40.
Pubmed: (View Article at PubMed) PMID:10936026
DOI: Full-text: DOI:10.1006/jaut.2000.0389

GPIIb/IIIa, the human platelet glycoprotein complex, is the autoantigen most commonly recognized by autoantibodies in autoimmune thrombocytopenic purpura (AITP). Two murine monoclonal antibodies (mAbs), namely Y2/51 and 5B12, directed against gpIIIa and gpIIb/IIIa, respectively, and rabbit anti-human platelet polyclonal antibodies have been used to select AITP-related epitopes from a phage display peptide library expressing random dodecapeptides in the pIII coat protein of M13 phage. The selected phage clones were tested by ELISA for binding to rabbit anti-human platelet antibodies as well as to sera from AITP patients. Seven clones reacted strongly with rabbit anti-human platelet antibodies, and four clones reacted with sera from AITP patients. Some homology between peptide inserts sequences of selected clones and human platelet gpIIIa and gpIb were found.


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CRRD Object Information
CRRD ID: 10755473
Created: 2016-01-28
Species: All species
Last Modified: 2016-01-28
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.