Differential control of mesangial cell proliferation by interferon-gamma.

Authors: Kakizaki, Y  Kraft, N  Atkins, RC 
Citation: Kakizaki Y, etal., Clin Exp Immunol. 1991 Jul;85(1):157-63.
Pubmed: (View Article at PubMed) PMID:1906384

Rat mesangial cells were shown to be sensitive to recombinant interferon-gamma (IFN-gamma). IFN-gamma reduced thymidine uptake by these cells and inhibited cell proliferation. Incubation of the cells with 1000 U/ml IFN-gamma decreased thymidine uptake by up to 64% and cell numbers were decreased by 17%. The effects of IFN-gamma were dose and time dependent and were partially reversible by the anti-IFN-gamma monoclonal antibody DB-1. This lymphokine did not reduce incorporation of RNA and protein precursors however. Measurements of 3H-uridine and 3H-leucine incorporation indicated significant increases in RNA and protein synthesis (37% and 45%, respectively) on a per cell basis. The mitogenic effects of IL-1 and platelet-derived growth factor (PDGF) were also susceptible to IFN-gamma-mediated inhibition but the mitogenic response to epidermal growth factor (EGF) was much less sensitive. We conclude that while IFN-gamma may act to modulate the mitogenic signals provided by some factors such as IL-1 and PDGF, the response to EGF appears to be unaffected.


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CRRD ID: 10755757
Created: 2016-02-04
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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.