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[Expression down-regulation of c-Cbl and Cbl-b genes in peripheral blood mononuclear cells from multiple myeloma patients].

Authors: Fang, S  Guo, X  Leng, X  Li, B  Niu, Y  Chen, S  Yang, L  Li, Y 
Citation: Fang S, etal., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Aug;29(8):842-5.
Pubmed: (View Article at PubMed) PMID:23948411

OBJECTIVE: To investigate c-Cbl and Cbl-b gene expressions in peripheral blood mononuclear cells (PBMCs) from multiple myeloma (MM) patients. METHODS: SYBR(R); Green PCR technique was used to detect c-Cbl and Cbl-b gene expressions in PBMCs from 23 MM patients and 22 healthy individuals, and RT-PCR and DNA sequence analysis were performed to analyze the mutations of 7-10 exons of c-Cbl. RESULTS: The expression of c-Cbl gene in MM patients (median: 0.798%) significantly decreased as compared with that in healthy controls (median: 2.443%) (P<0.05). The expression of Cbl-b gene in MM patients (median: 0.714%) also dropped significantly as compared with that in healthy controls (median: 2.179%) (P<0.05). The 7-10 exons of c-Cbl gene had two different sizes of fragments in 2 MM patients: 483 bp and 148 bp which were wild-type and deletion mutants type of c-Cbl gene. c-Cbl gene mutations were not found in all MM patients. CONCLUSION: The expressions of c-Cbl and Cbl-b genes in PBMCs from MM patients are down-regulated.

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CRRD Object Information
CRRD ID: 11038794
Created: 2016-02-25
Species: All species
Last Modified: 2016-02-25
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.