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Role of the PAR-3-KIF3 complex in the establishment of neuronal polarity.

Authors: Nishimura, T  Kato, K  Yamaguchi, T  Fukata, Y  Ohno, S  Kaibuchi, K 
Citation: Nishimura T, etal., Nat Cell Biol. 2004 Apr;6(4):328-34. Epub 2004 Mar 28.
Pubmed: (View Article at PubMed) PMID:15048131
DOI: Full-text: DOI:10.1038/ncb1118

Neurons polarize to form elaborate multiple dendrites and one long axon. The establishment and maintenance of axon/dendrite polarity are fundamentally important for neurons. Recent studies have demonstrated that the polarity complex PAR-3-PAR-6-atypical protein kinase C (aPKC) is involved in polarity determination in many tissues and cells. The function of the PAR-3-PAR-6-aPKC protein complex depends on its subcellular localization in polarized cells. PAR-3 accumulates at the tip of growing axons in cultured rat hippocampal neurons, but the molecular mechanism of this localization remains unknown. Here we identify a direct interaction between PAR-3 and KIF3A, a plus-end-directed microtubule motor protein, and show that aPKC can associate with KIF3A through its interaction with PAR-3. The expression of dominant-negative PAR-3 and KIF3A fragments that disrupt PAR-3-KIF3A binding inhibited the accumulation of PAR-3 and aPKC at the tip of the neurites and abolished neuronal polarity. These results suggest that PAR-3 is transported to the distal tip of the axon by KIF3A and that the proper localization of PAR-3 is required to establish neuronal polarity.

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CRRD Object Information
CRRD ID: 11049589
Created: 2016-04-11
Species: All species
Last Modified: 2016-04-11
Status: ACTIVE



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