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PKC412 inhibits the zinc finger 198-fibroblast growth factor receptor 1 fusion tyrosine kinase and is active in treatment of stem cell myeloproliferative disorder.

Authors: Chen, J  DeAngelo, DJ  Kutok, JL  Williams, IR  Lee, BH  Wadleigh, M  Duclos, N  Cohen, S  Adelsperger, J  Okabe, R  Coburn, A  Galinsky, I  Huntly, B  Cohen, PS  Meyer, T  Fabbro, D  Roesel, J  Banerji, L  Griffin, JD  Xiao, S  Fletcher, JA  Stone, RM  Gilliland, DG 
Citation: Chen J, etal., Proc Natl Acad Sci U S A. 2004 Oct 5;101(40):14479-84. Epub 2004 Sep 24.
Pubmed: (View Article at PubMed) PMID:15448205
DOI: Full-text: DOI:10.1073/pnas.0404438101

Human stem cell leukemia-lymphoma syndrome usually presents itself as a myeloproliferative disorder (MPD) that evolves to acute myeloid leukemia and/or lymphoma. The syndrome associated with t(8;13)(p11;q12) results in expression of the ZNF198-fibroblast growth factor receptor (FGFR) 1 fusion tyrosine kinase. Current empirically derived cytotoxic chemotherapy is inadequate for treatment of this disease. We hypothesized that small-molecule inhibitors of the ZNF198-FGFR1 fusion would have therapeutic efficacy. We characterized the transforming activity of ZNF198-FGFR1 in hematopoietic cells in vitro and in vivo. Expression of ZNF198-FGFR1 in primary murine hematopoietic cells caused a myeloproliferative syndrome in mice that recapitulated the human MPD phenotype. Transformation in these assays, and activation of the downstream effector molecules PLC-gamma, STAT5, and phosphatidylinositol 3-kinase/AKT, required the proline-rich domains, but not the ZNF domains, of ZNF198. A small-molecule tyrosine kinase inhibitor, PKC412 (N-benzoyl-staurosporine) effectively inhibited ZNF198-FGFR1 tyrosine kinase activity and activation of downstream effector pathways, and inhibited proliferation of ZNF198-FGFR1 transformed Ba/F3 cells. Furthermore, treatment with PKC412 resulted in statistically significant prolongation of survival in the murine model of ZNF198-FGFR1-induced MPD. Based in part on these data, PKC412 was administered to a patient with t(8;13)(p11;q12) and was efficacious in treatment of progressive myeloproliferative disorder with organomegaly. Therefore, PKC412 may be a useful therapy for treatment of human stem cell leukemia-lymphoma syndrome.

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CRRD Object Information
CRRD ID: 11352309
Created: 2016-07-13
Species: All species
Last Modified: 2016-07-13
Status: ACTIVE



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