Submit Data |  Help |  Video Tutorials |  News |  Publications |  FTP Download |  REST API |  Citing RGD |  Contact   

Expression and colocalization of beta-catenin and lymphoid enhancing factor-1 in prostate cancer progression.

Authors: Bauman, TM  Vezina, CM  Ricke, EA  Halberg, RB  Huang, W  Peterson, RE  Ricke, WA 
Citation: Bauman TM, etal., Hum Pathol. 2016 May;51:124-33. doi: 10.1016/j.humpath.2015.12.024. Epub 2016 Jan 19.
Pubmed: (View Article at PubMed) PMID:27067790
DOI: Full-text: DOI:10.1016/j.humpath.2015.12.024

The purpose of this study was to objectively investigate beta-catenin and LEF1 abundance, subcellular localization, and colocalization across benign and staged prostate cancer (PCa) specimens. A tissue microarray containing tumor-adjacent histologically benign prostate tissue (BPT; n = 48 patients), high-grade prostatic intraepithelial neoplasia (HGPIN; n = 25), localized PCa (n = 42), aggressive PCa (n = 31), and metastases (n = 22) was stained using multiplexed immunohistochemistry with antibodies toward E-cadherin, beta-catenin, and LEF1. Multispectral imaging was used for quantitation, and protein expression and colocalization was evaluated across PCa progression. Stromal nuclear beta-catenin abundance was greater in HGPIN and PCa compared with BPT (P < .05 for both), and epithelial nuclear beta-catenin abundance was lower in metastatic PCa than in BPT (P < .05 for both). Epithelial and stromal nuclear LEF1 abundance was greater in HGPIN compared with BPT, whereas epithelial nuclear LEF1 was also greater in metastases. The proportion of epithelial and stromal nuclear double-positive beta-catenin(+)/LEF1(+) cells was greater in HGPIN compared with BPT. In addition, the proportion of epithelial beta-catenin(+)/LEF1(+) cells was greater in localized PCa and metastases compared with BPT. A significant amount of stromal cells were positive for LEF1 but not beta-catenin. beta-Catenin and LEF1 abundance were negatively correlated in the epithelium (P < .0001) but not the stroma (P > .05). We conclude that beta-catenin and LEF1 colocalization is increased in HGPIN and metastasis relative to BPT, suggesting a role for beta-catenin/LEF1-mediated transcription in both malignant transformation and metastasis of PCa. Furthermore, our results suggest that LEF1 abundance alone is not a reliable readout for beta-catenin activity in prostate tissues.


Disease Annotations
Objects Annotated

Additional Information

CRRD Object Information
CRRD ID: 11531513
Created: 2016-09-02
Species: All species
Last Modified: 2016-09-02
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.