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[Quantification of the PRAME transcripts in patients with acute myeloid leukemia].

Authors: Zhu, Zhao-hui  Qian, Jun  Lin, Jiang  Yao, Dong-ming  Qian, Zhen  Wang, Ya-li  Chen, Qin  Han, Lan-xiu  Xiao, Gaofei 
Citation: Zhu ZH, etal., Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2010 Apr;27(2):149-52. doi: 10.3760/cma.j.issn.1003-9406.2010.02.007.
Pubmed: (View Article at PubMed) PMID:20376794
DOI: Full-text: DOI:10.3760/cma.j.issn.1003-9406.2010.02.007

OBJECTIVE: To analyze the expression level and clinical significance of the preferentially expressed antigen of melanoma (PRAME) transcripts in patients with acute myeloid leukemia (AML).
METHODS: Real-time quantitative polymerase chain reaction with EvaGreen dye was established to detect the expression level of PRAME transcripts in the bone marrow mononuclear cells of 56 AML cases and 20 controls. The clinical association of PRAME transcripts was analyzed.
RESULTS: The PRAME transcripts were 0-1.46% (median 0.18%) and 0-21 618.09% (median 9.79%) in controls and AML cases, respectively (P< 0.01). Among the FAB subtypes, those with M1, M2, M3 and M4 had significantly higher level of PRAME transcripts than controls. However, those with M5 had similar level of PRAME transcripts as controls. There was a significantly negative correlation between the PRAME transcripts and cytogenetic risk groups (r= -0.438, P= 0.001). Cases in low risk had significantly higher level of PRAME transcripts than those in intermediate and high risk. Among cases with AML-M2, those with t(8;21) had significantly higher level of PRAME transcripts (135.06% -21 618.09%, median 2201.88%) than those without t(8;21)(0.14% -1696.30%, median 17.97%)(P= 0.002). In a patient with sequential samples, PRAME transcripts significantly decreased after induction therapy and significantly increased after relapse.
CONCLUSION: The PRAME transcript was highly expressed in AML patients and was a favorable marker of prognosis. Quantification of PRAME transcript can be used in monitoring disease status of AML.


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CRRD Object Information
CRRD ID: 11535023
Created: 2016-09-19
Species: All species
Last Modified: 2017-01-03
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.