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ARP1 in Golgi organisation and attachment of manchette microtubules to the nucleus during mammalian spermatogenesis.

Authors: Fouquet, J  Kann, M  Soues, S  Melki, R 
Citation: Fouquet J, etal., J Cell Sci. 2000 Mar;113 ( Pt 5):877-86.
Pubmed: (View Article at PubMed) PMID:10671377

Actin related protein of vertebrate, Arp1, is a major component of the dynactin complex. To characterise and localise Arp1 during mammalian spermatogenesis, polyclonal antibodies were raised against a human recombinant Arp1. Anti-Arp1 antibodies were used for western-immunoblotting, indirect immunofluorescence and immunoelectron microscopy. In round spermatids, Arp1 was detected at the centrosome and at the Golgi apparatus. In elongated spermatids, Arp1 was predominantly found along microtubules of the manchette and at their site of attachment to the nuclear envelope. In maturing spermatids, Arp1 was still present in the pericentriolar material, but in testicular spermatozoa it was not detectable. These various localisations of Arp1 and their changes during spermatid differentiation suggest that the dynactin complex in association with dynein might contribute to several activities: the functional organisation of the centrosome and of the Golgi apparatus and the shaping of the nucleus by manchette microtubules.


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CRRD Object Information
CRRD ID: 11541119
Created: 2016-10-10
Species: All species
Last Modified: 2016-10-10
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.