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Naofen, a novel WD40-repeat protein, mediates spontaneous and tumor necrosis factor-induced apoptosis.

Authors: Feng, GG  Li, C  Huang, L  Tsunekawa, K  Sato, Y  Fujiwara, Y  Komatsu, T  Honda, T  Fan, JH  Goto, H  Koide, T  Hasegawa, T  Ishikawa, N 
Citation: Feng GG, etal., Biochem Biophys Res Commun. 2010 Mar 26;394(1):153-7. doi: 10.1016/j.bbrc.2010.02.133. Epub 2010 Mar 1.
Pubmed: (View Article at PubMed) PMID:20193664
DOI: Full-text: DOI:10.1016/j.bbrc.2010.02.133

Naofen has recently been identified from the rat brain/spinal cord cDNA library as a substance reactive against an anti-shigatoxin (Stx)-2 antibody. Naofen mRNA is composed of 4620 nucleotides and encodes 1170 amino acids. Naofen contains four WD-repeat domains in its N-terminus and is ubiquitously distributed in many tissues of the rat. Tumor necrosis factor (TNF)-alpha enhanced the expression of naofen mRNA in HEK293 cells in a dose-dependent manner. Furthermore, naofen siRNA, which predominantly knocked down the expression of naofen mRNA, significantly reduced both TNF-alpha-induced caspase-3 activation and apoptosis in HEK293 cells. Overexpression of naofen in HEK293 cells (FLAG-NF) spontaneously induced caspase -3 activation and apoptosis, and showed extremely high susceptibility to TNF-alpha-induced apoptosis. These results indicated that naofen may function as a novel modulator activating caspase-3, and promoting TNF-alpha-stimulated apoptosis.

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CRRD Object Information
CRRD ID: 11553917
Created: 2016-10-17
Species: All species
Last Modified: 2016-10-17
Status: ACTIVE



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