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TGF-beta 1 modulation of IGF-I signaling pathway in rat thyroid epithelial cells.

Authors: Mincione, G  Esposito, DL  Di Marcantonio, MC  Piccirelli, A  Cama, A  Colletta, G 
Citation: Mincione G, etal., Exp Cell Res. 2003 Jul 15;287(2):411-23.
Pubmed: (View Article at PubMed) PMID:12837295

Transforming growth factor beta 1 (TGF-beta 1) and insulin-like growth factor I (IGF-I) have contrasting effects on cell cycle regulation in thyroid cells and TGF-beta 1 induces a dramatic decrease in IGF-I-induced cell proliferation. The aim of the present study was to investigate the molecular mechanism of cross-talk between TGF-beta 1 and IGF-I in FRTL-5 cells. TGF-beta 1 affected IGF-I-stimulated insulin receptor substrate 1 (IRS-1) tyrosine phosphorylation and its association with Grb2 protein. Moreover, TGF-beta 1 decreased the IGF-I-induced tyrosine phosphorylation of the adaptor protein CrkII and its association with the IGF-I receptor. These results were accompanied by TGF-beta 1 inhibition of IGF-I-stimulated mitogen-activated protein kinase phosphorylation and activation. Conversely, TGF-beta 1 did not alter IGF-I-stimulated IRS-1-associated phosphatidylinositol 3-kinase activity, IGF-I-induced tyrosine phosphorylation of Shc, and its binding to Grb2. Taken together, these findings provide a molecular basis for the growth-inhibitory action of TGF-beta 1 on the IGF-I-induced mitogenic effect.


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CRRD Object Information
CRRD ID: 11568682
Created: 2016-12-12
Species: All species
Last Modified: 2016-12-12
Status: ACTIVE


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