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UTP affects the Schwannoma cell line proteome through P2Y receptors leading to cytoskeletal reorganisation.

Authors: Martiáñez, Tánia  Carrascal, Montserrat  Lamarca, Aloa  Segura, Mònica  Durany, Núria  Masgrau, Roser  Abian, Joaquín  Gella, Alejandro 
Citation: Martiáñez T, etal., Proteomics. 2012 Jan;12(1):145-56. doi: 10.1002/pmic.201100187. Epub 2011 Dec 9.
Pubmed: (View Article at PubMed) PMID:22065602
DOI: Full-text: DOI:10.1002/pmic.201100187

Glial cells in the peripheral nervous system, such as Schwann cells, respond to nucleotides, which play an important role in axonal regeneration and myelination. Metabotropic P2Y receptor agonists are promising therapeutic molecules for peripheral neuropathies. Nevertheless, the proteomic mechanisms involved in nucleotide action on Schwann cells remain unknown. Here, we studied intracellular protein changes in RT4-D6P2T Schwann cells after treatment with nucleotides and Nucleo CMP Forte (CMPF), a nucleotide-based drug. After treatment with CMPF, 2-D DIGE revealed 11 differential gel spots, which were all upregulated. Among these, six different proteins were identified by MS. Some of these proteins are involved in actin remodelling (actin-related protein, Arp3), membrane vesicle transport (Rab GDP dissociation inhibitor ß, Rab GDI), and the endoplasmic reticulum stress response (protein disulfide isomerase A3, PDI), which are hallmarks of a possible P2Y receptor signalling pathway. Expression of P2Y receptors in RT4-D6P2T cells was demonstrated by RT-PCR and a transient elevation of intracellular calcium measured in response to UTP. Actin reorganisation was visualized after UTP treatment using phalloidin-FITC staining and was blocked by the P2Y antagonist suramin, which also inhibited Arp3, Rab GDI, and PDI protein upregulation. Our data indicate that extracellular UTP interacts with Schwann P2Y receptors and activates molecular machinery that induces changes in the glial cell cytoskeleton.

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CRRD Object Information
CRRD ID: 11571625
Created: 2016-12-21
Species: All species
Last Modified: 2016-12-21
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.