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A WASp-VASP complex regulates actin polymerization at the plasma membrane.

Authors: Castellano, F  Le Clainche, C  Patin, D  Carlier, M F  Chavrier, P 
Citation: Castellano F, etal., EMBO J. 2001 Oct 15;20(20):5603-14.
Pubmed: (View Article at PubMed) PMID:11598004
DOI: Full-text: DOI:10.1093/emboj/20.20.5603

Proteins of the Wiskott-Aldrich syndrome and Ena/VASP families both play essential functions in the regulation of actin dynamics at the cell leading edge. However, possibilities of functional interplay between members of these two families have not been addressed. Here we show that, in hemopoietic cells, recruitment of the C-terminal VCA (Verprolin homology, Cofilin homology, Acidic) domain of WASp at the plasma membrane by a ligand technique using rapamycin as an intermediate is not sufficient to elicit efficient Arp2/3 complex-mediated actin polymerization. Other domains of WASp, in particular the proline-rich domain, are required for the formation of actin-rich structures. An in vitro analysis demonstrates that the proline-rich domain of WASp binds VASP with an affinity of approximately 10(6) M(-1). In addition, WASp and VASP both accumulate in actin-rich phagocytic cups. Finally, in a reconstituted motility medium, VASP enhances actin-based propulsion of WASp-coated beads in a fashion reminiscent of its effect on Listeria movement. We propose that VASP and WASp cooperation is essential in stimulating actin assembly and membrane protrusion at the leading edge.

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CRRD Object Information
CRRD ID: 11576278
Created: 2017-01-03
Species: All species
Last Modified: 2017-01-03
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.