Submit Data |  Help |  Video Tutorials |  News |  Publications |  FTP Download |  REST API |  Citing RGD |  Contact   

Molecular anatomy of a trafficking organelle.

Authors: Takamori, Shigeo  Holt, Matthew  Stenius, Katinka  Lemke, Edward A  Grønborg, Mads  Riedel, Dietmar  Urlaub, Henning  Schenck, Stephan  Brügger, Britta  Ringler, Philippe  Müller, Shirley A  Rammner, Burkhard  Gräter, Frauke  Hub, Jochen S  De Groot, Bert L  Mieskes, Gottfried  Moriyama, Yoshinori  Klingauf, Jürgen  Grubmüller, Helmut  Heuser, John  Wieland, Felix  Jahn, Reinhard 
Citation: Takamori S, etal., Cell. 2006 Nov 17;127(4):831-46.
Pubmed: (View Article at PubMed) PMID:17110340
DOI: Full-text: DOI:10.1016/j.cell.2006.10.030

Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor compartment and fuse with an acceptor compartment. Common principles of budding and fusion have emerged, and many of the proteins involved in these events are now known. However, a detailed picture of an entire trafficking organelle is not yet available. Using synaptic vesicles as a model, we have now determined the protein and lipid composition; measured vesicle size, density, and mass; calculated the average protein and lipid mass per vesicle; and determined the copy number of more than a dozen major constituents. A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins. Synaptic vesicles are dominated by proteins, possess a surprising diversity of trafficking proteins, and, with the exception of the V-ATPase that is present in only one to two copies, contain numerous copies of proteins essential for membrane traffic and neurotransmitter uptake.

Annotation

Gene Ontology Annotations
Objects Annotated

Additional Information

 
CRRD Object Information
CRRD ID: 12050113
Created: 2017-01-21
Species: All species
Last Modified: 2017-01-21
Status: ACTIVE



NHLBI Logo

RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.