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Molecular anatomy of a trafficking organelle.

Authors: Takamori, Shigeo  Holt, Matthew  Stenius, Katinka  Lemke, Edward A  Grønborg, Mads  Riedel, Dietmar  Urlaub, Henning  Schenck, Stephan  Brügger, Britta  Ringler, Philippe  Müller, Shirley A  Rammner, Burkhard  Gräter, Frauke  Hub, Jochen S  De Groot, Bert L  Mieskes, Gottfried  Moriyama, Yoshinori  Klingauf, Jürgen  Grubmüller, Helmut  Heuser, John  Wieland, Felix  Jahn, Reinhard 
Citation: Takamori S, etal., Cell. 2006 Nov 17;127(4):831-46.
Pubmed: (View Article at PubMed) PMID:17110340
DOI: Full-text: DOI:10.1016/j.cell.2006.10.030

Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor compartment and fuse with an acceptor compartment. Common principles of budding and fusion have emerged, and many of the proteins involved in these events are now known. However, a detailed picture of an entire trafficking organelle is not yet available. Using synaptic vesicles as a model, we have now determined the protein and lipid composition; measured vesicle size, density, and mass; calculated the average protein and lipid mass per vesicle; and determined the copy number of more than a dozen major constituents. A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins. Synaptic vesicles are dominated by proteins, possess a surprising diversity of trafficking proteins, and, with the exception of the V-ATPase that is present in only one to two copies, contain numerous copies of proteins essential for membrane traffic and neurotransmitter uptake.


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CRRD Object Information
CRRD ID: 12050113
Created: 2017-01-21
Species: All species
Last Modified: 2017-01-21
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.