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Differential vesicular sorting of AMPA and GABAA receptors.

Authors: Gu, Yi  Chiu, Shu-Ling  Liu, Bian  Wu, Pei-Hsun  Delannoy, Michael  Lin, Da-Ting  Wirtz, Denis  Huganir, Richard L 
Citation: Gu Y, etal., Proc Natl Acad Sci U S A. 2016 Feb 16;113(7):E922-31. doi: 10.1073/pnas.1525726113. Epub 2016 Feb 2.
Pubmed: (View Article at PubMed) PMID:26839408
DOI: Full-text: DOI:10.1073/pnas.1525726113

In mature neurons AMPA receptors cluster at excitatory synapses primarily on dendritic spines, whereas GABAA receptors cluster at inhibitory synapses mainly on the soma and dendritic shafts. The molecular mechanisms underlying the precise sorting of these receptors remain unclear. By directly studying the constitutive exocytic vesicles of AMPA and GABAA receptors in vitro and in vivo, we demonstrate that they are initially sorted into different vesicles in the Golgi apparatus and inserted into distinct domains of the plasma membrane. These insertions are dependent on distinct Rab GTPases and SNARE complexes. The insertion of AMPA receptors requires SNAP25-syntaxin1A/B-VAMP2 complexes, whereas insertion of GABAA receptors relies on SNAP23-syntaxin1A/B-VAMP2 complexes. These SNARE complexes affect surface targeting of AMPA or GABAA receptors and synaptic transmission. Our studies reveal vesicular sorting mechanisms controlling the constitutive exocytosis of AMPA and GABAA receptors, which are critical for the regulation of excitatory and inhibitory responses in neurons.

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CRRD Object Information
CRRD ID: 12050145
Created: 2017-01-21
Species: All species
Last Modified: 2017-01-21
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.