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Short hairpin ribonucleic acid constructs targeting insulin-like growth factor binding protein-3 rehabilitated decreased testosterone concentrations in diabetic rats.

Authors: Zhou, Zhang-Yan  Li, Fei  Cheng, Shao-Ping  Huang, Hui  Peng, Bi-Wen  Wang, Jing  Liu, Chang-Mao  Xing, Cheng  Sun, Ya-Ling  Bsoul, Najeeb  Pan, Hui  Yi, Cun-Jian  Liu, Rong-Hua  Zhong, Guang-Jun 
Citation: Zhou ZY, etal., Med Sci Monit. 2015 Jan 8;21:94-9. doi: 10.12659/MSM.891382.
Pubmed: (View Article at PubMed) PMID:25582342
DOI: Full-text: DOI:10.12659/MSM.891382


BACKGROUND: The aim of this study was to determine if shRNA constructs targeting insulin-like growth factor binding protein-3 can rehabilitate decreased serum testosterone concentrations in streptozotocin-induced diabetic rats.
MATERIAL/METHODS: After 12 weeks of intracavernous administration of IGFBP-3 shRNA, intracavernous pressure responses to electrical stimulation of cavernous nerves were evaluated. The expression of IGFBP-3 at mRNA and protein levels was detected by quantitative real-time PCR analysis and Western blot, respectively. The concentrations of serum testosterone and cavernous cyclic guanosine monophosphate were detected by enzyme-linked immunosorbent assay.
RESULTS: After 12 weeks of intracavernous administration of IGFBP-3 shRNA, the cavernosal pressure was significantly increased in response to the cavernous nerves stimulation compared to the diabetic control group (p<0.01). Cavernous IGFBP-3 expression at both mRNA and protein levels was significantly inhibited. Both serum testosterone and cavernous cyclic guanosine monophosphate concentrations were significantly increased in the IGFBP-3 shRNA treatment group compared to the diabetic control group (p<0.01).
CONCLUSIONS: These results suggest that IGFBP-3 shRNA may rehabilitate erectile function via increases of concentrations of serum testosterone and cavernous cyclic guanosine monophosphate in streptozotocin-induced diabetic rats.

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CRRD Object Information
CRRD ID: 12743617
Created: 2017-02-10
Species: All species
Last Modified: 2017-02-10
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.