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Synaptic control of mRNA translation by reversible assembly of XRN1 bodies.

Authors: Luchelli, Luciana  Thomas, MarĂ­a Gabriela  Boccaccio, Graciela L 
Citation: Luchelli L, etal., J Cell Sci. 2015 Apr 15;128(8):1542-54. doi: 10.1242/jcs.163295. Epub 2015 Mar 3.
Pubmed: (View Article at PubMed) PMID:25736288
DOI: Full-text: DOI:10.1242/jcs.163295

Repression of mRNA translation is linked to the formation of specific cytosolic foci such as stress granules and processing bodies, which store or degrade mRNAs. In neurons, synaptic activity regulates translation at the post-synapse and this is important for plasticity. N-methyl-D-aspartate (NMDA) receptor stimulation downregulates translation, and we speculate that this is linked to the formation of unknown mRNA-silencing foci. Here, we show that the 5'-3' exoribonuclease XRN1 forms discrete clusters associated with the post-synapse that are different from processing bodies or stress granules, and we named them synaptic XRN1 bodies (SX-bodies). Using primary neurons, we found that the SX-bodies respond to synapse stimulation and that their formation correlates inversely with the local translation rate. SX-bodies increase in size and number upon NMDA stimulation, and metabotropic glutamate receptor activation provokes SX-body dissolution, along with increased translation. The response is specific and the previously described Smaug1 foci and FMRP granules show a different response. Finally, XRN1 knockdown impairs the translational repression triggered by NMDA. Collectively, these observations support a role for the SX-bodies in the reversible masking and silencing of mRNAs at the synapse.

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CRRD Object Information
CRRD ID: 12791033
Created: 2017-02-28
Species: All species
Last Modified: 2017-02-28
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.