The RNA-binding protein MARTA2 regulates dendritic targeting of MAP2 mRNAs in rat neurons.

Authors: Zivraj, Krishna H  Rehbein, Monika  Ölschläger-Schütt, Janin  Schob, Claudia  Falley, Katrin  Buck, Friedrich  Schweizer, Michaela  Schepis, Antonino  Kremmer, Elisabeth  Richter, Dietmar  Kreienkamp, Hans-Jürgen  Kindler, Stefan 
Citation: Zivraj KH, etal., J Neurochem. 2013 Mar;124(5):670-84. doi: 10.1111/jnc.12079. Epub 2013 Jan 20.
Pubmed: (View Article at PubMed) PMID:23121659
DOI: Full-text: DOI:10.1111/jnc.12079

Dendritic targeting of mRNAs encoding the microtubule-associated protein 2 (MAP2) in neurons involves a cis-acting dendritic targeting element. Two rat brain proteins, MAP2-RNA trans-acting protein (MARTA)1 and MARTA2, bind to the cis-element with both high affinity and specificity. In this study, affinity-purified MARTA2 was identified as orthologue of human far-upstream element binding protein 3. In neurons, it resides in somatodendritic granules and dendritic spines and associates with MAP2 mRNAs. Expression of a dominant-negative variant of MARTA2 disrupts dendritic targeting of endogenous MAP2 mRNAs, while not noticeably altering the level and subcellular distribution of polyadenylated mRNAs as a whole. Finally, MAP2 transcripts associate with the microtubule-based motor KIF5 and inhibition of KIF5, but not cytoplasmic dynein function disrupts extrasomatic trafficking of MAP2 mRNA granules. Thus, in neurons MARTA2 appears to represent a key trans-acting factor involved in KIF5-mediated dendritic targeting of MAP2 mRNAs.

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CRRD ID: 12792285
Created: 2017-03-15
Species: All species
Last Modified: 2017-03-15
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.