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Doublecortin (DCX) immunoreactivity in hippocampus of chronic refractory temporal lobe epilepsy patients with hippocampal sclerosis.

Authors: D'Alessio, Luciana  Konopka, Hector  López, Ester María  Seoane, Eduardo  Consalvo, Damián  Oddo, Silvia  Kochen, Silvia  López-Costa, Juan José 
Citation: D'Alessio L, etal., Seizure. 2010 Nov;19(9):567-72. doi: 10.1016/j.seizure.2010.09.004.
Pubmed: (View Article at PubMed) PMID:20888264
DOI: Full-text: DOI:10.1016/j.seizure.2010.09.004

INTRODUCTION: Status epilepticus increases the production of new neurons (hippocampal neurogenesis) and promotes aberrant migration. However chronic experimental models of epilepsy and studies performed in human epilepsy showed controversial results suggesting a reduction in hippocampal neurogenesis in late stages of the disease. Doublecortin (DCX) has been validated to determine alterations in the production of new neurons in the human hippocampus.
OBJECTIVES: Determine DCX expression in human hippocampal sclerosis (HS) from patients who underwent epilepsy surgery for refractory temporal lobe epilepsy (TLE).
METHODS: Hippocampal sections of 9 patients with HS and TLE who underwent surgery, were processed using immunoperoxidase for DCX. Archival material from 5 normal post-mortem hippocampus were simultaneously processed.
RESULTS: Significantly lower staining intensity was observed in DCX-positive neurons localized in dentate gyrus (DG) and in CA1 of epileptic hippocampus; lower DCX reactive area was observed in pyramidal layers of CA1; and a reduced in the mean number of DCX-positive neurons were determined in DG compared to normal hippocampus (p<0.05).
CONCLUSIONS: This study found a decrease in DCX expression in hippocampus of patients with HS and chronic and refractory TLE suggesting alterations in NG and hippocampal synaptogenesis with potential cognitive and emotional repercussion.


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CRRD Object Information
CRRD ID: 12904713
Created: 2017-05-18
Species: All species
Last Modified: 2017-05-18
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.