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Cloning and expression of cDNA encoding 25-hydroxyvitamin D3 24-hydroxylase.

Authors: Ohyama, Y  Noshiro, M  Okuda, K 
Citation: Ohyama Y, etal., FEBS Lett 1991 Jan 28;278(2):195-8.
Pubmed: (View Article at PubMed) PMID:1991512

A cDNA encoding 25-hydroxyvitamin D3 24-hydroxylase (P450cc24) was isolated from a rat kidney cDNA library using specific antibodies to the enzyme. The isolated cDNA was 3.2 kbp long and contained a 1542-bp open reading frame encoding 514 amino acids. The deduced amino acid sequence contained a presequence typical of mitochondrial enzymes in the N-terminal region. The amino acid sequence shows less than 30% similarity to those of any other cytochrome P450s so far reported and, therefore, P450cc24 constitutes a novel family of P450. COS-7 cells transfected with the cDNA produced a protein that was reactive with the antibodies and catalyzed NADPH-dependent 24-hydroxylation of 25-hydroxyvitamin D3 in the presence of adrenodoxin and NADPH-adrenodoxin reductase. Using the cDNA as a probe we demonstrated that the increase of 24-hydroxylation activity caused by administration of vitamin D3 into rats was accompanied by an increase of the mRNA.

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CRRD Object Information
CRRD ID: 1298844
Created: 2004-06-01
Species: All species
Last Modified: 2006-04-25
Status: ACTIVE



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