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Characterization of SMOC-1, a novel modular calcium-binding protein in basement membranes.

Authors: Vannahme, C  Smyth, N  Miosge, N  Gosling, S  Frie, C  Paulsson, M  Maurer, P  Hartmann, U 
Citation: Vannahme C, etal., J Biol Chem 2002 Oct 11;277(41):37977-86. Epub 2002 Jul 18.
Pubmed: (View Article at PubMed) PMID:12130637
DOI: Full-text: DOI:10.1074/jbc.M203830200

We have isolated the novel gene SMOC-1 that encodes a secreted modular protein containing an EF-hand calcium-binding domain homologous to that in BM-40. It further consists of two thyroglobulin-like domains, a follistatin-like domain and a novel domain. Recombinant expression in human cells showed that SMOC-1 is a glycoprotein with a calcium-dependent conformation. Results from Northern blots, reverse transcriptase-PCR, and immunoblots revealed a widespread expression in many tissues. Immunofluorescence studies with an antiserum directed against recombinant human SMOC-1 demonstrated a basement membrane localization of the protein and additionally its presence in other extracellular matrices. Immunogold electron microscopy confirmed the localization of SMOC-1 within basement membranes in kidney and skeletal muscle as well as its expression in the zona pellucida surrounding the oocyte.


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CRRD Object Information
CRRD ID: 1331355
Created: 2005-01-17
Species: All species
Last Modified: 2005-01-17
Status: ACTIVE


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