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Microtubule minus-end binding protein CAMSAP2 controls axon specification and dendrite development.

Authors: Yau, Kah Wai  van Beuningen, Sam F B  Cunha-Ferreira, Inês  Cloin, Bas M C  van Battum, Eljo Y  Will, Lena  Schätzle, Philipp  Tas, Roderick P  van Krugten, Jaap  Katrukha, Eugene A  Jiang, Kai  Wulf, Phebe S  Mikhaylova, Marina  Harterink, Martin  Pasterkamp, R Jeroen  Akhmanova, Anna  Kapitein, Lukas C  Hoogenraad, Casper C 
Citation: Yau KW, etal., Neuron. 2014 Jun 4;82(5):1058-73. doi: 10.1016/j.neuron.2014.04.019.
Pubmed: (View Article at PubMed) PMID:24908486
DOI: Full-text: DOI:10.1016/j.neuron.2014.04.019

In neurons, most microtubules are not associated with a central microtubule-organizing center (MTOC), and therefore, both the minus and plus-ends of these non-centrosomal microtubules are found throughout the cell. Microtubule plus-ends are well established as dynamic regulatory sites in numerous processes, but the role of microtubule minus-ends has remained poorly understood. Using live-cell imaging, high-resolution microscopy, and laser-based microsurgery techniques, we show that the CAMSAP/Nezha/Patronin family protein CAMSAP2 specifically localizes to non-centrosomal microtubule minus-ends and is required for proper microtubule organization in neurons. CAMSAP2 stabilizes non-centrosomal microtubules and is required for neuronal polarity, axon specification, and dendritic branch formation in vitro and in vivo. Furthermore, we found that non-centrosomal microtubules in dendrites are largely generated by γ-Tubulin-dependent nucleation. We propose a two-step model in which γ-Tubulin initiates the formation of non-centrosomal microtubules and CAMSAP2 stabilizes the free microtubule minus-ends in order to control neuronal polarity and development.


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CRRD Object Information
CRRD ID: 13441206
Created: 2017-10-24
Species: All species
Last Modified: 2017-10-24
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.