XLMR protein related to neurite extension (Xpn/KIAA2022) regulates cell-cell and cell-matrix adhesion and migration.

Authors: Magome, Takuya  Hattori, Tsuyoshi  Taniguchi, Manabu  Ishikawa, Toshiko  Miyata, Shingo  Yamada, Kohei  Takamura, Hironori  Matsuzaki, Shinsuke  Ito, Akira  Tohyama, Masaya  Katayama, Taiichi 
Citation: Magome T, etal., Neurochem Int. 2013 Nov;63(6):561-9. doi: 10.1016/j.neuint.2013.09.011. Epub 2013 Sep 24.
Pubmed: (View Article at PubMed) PMID:24071057
DOI: Full-text: DOI:10.1016/j.neuint.2013.09.011

X-linked mental retardation (XLMR) is a common cause of moderate to severe intellectual disability in males. XLMR protein related to neurite extension (Xpn, also known as KIAA2022) has been implicated as a gene responsible for XLMR in humans. Although Xpn is highly expressed in the developing brain and is involved in neurite outgrowth in PC12 cells and neurons, little is known about the functional role of Xpn. Here, we show that Xpn regulates cell-cell and cell-matrix adhesion and migration in PC12 cells. Xpn knockdown enhanced cell-cell and cell-matrix adhesion mediated by N-cadherin and ß1-integrin, respectively. N-Cadherin and ß1-integrin expression at the mRNA and protein levels was significantly increased in Xpn knockdown PC12 cells. Furthermore, overexpressed Xpn protein was strongly expressed in the nuclei of PC12 and 293T cells. Finally, depletion of Xpn perturbed cellular migration by enhancing N-cadherin and ß1-integrin expression in a PC12 cell wound healing assay. We conclude that Xpn regulates cell-cell and cell-matrix adhesion and cellular migration by regulating the expression of adhesion molecules.

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CRRD ID: 13442493
Created: 2017-11-06
Species: All species
Last Modified: 2017-11-06
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.