The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54.

Authors: Kotani, M  Detheux, M  Vandenbogaerde, A  Communi, D  Vanderwinden, JM  Le Poul, E  Brezillon, S  Tyldesley, R  Suarez-Huerta, N  Vandeput, F  Blanpain, C  Schiffmann, SN  Vassart, G  Parmentier, M 
Citation: Kotani M, etal., J Biol Chem 2001 Sep 14;276(37):34631-6. Epub 2001 Jul 16.
Pubmed: (View Article at PubMed) PMID:11457843
DOI: Full-text: DOI:10.1074/jbc.M104847200

Natural peptides displaying agonist activity on the orphan G protein-coupled receptor GPR54 were isolated from human placenta. These 54-, 14,- and 13-amino acid peptides, with a common RF-amide C terminus, derive from the product of KiSS-1, a metastasis suppressor gene for melanoma cells, and were therefore designated kisspeptins. They bound with low nanomolar affinities to rat and human GPR54 expressed in Chinese hamster ovary K1 cells and stimulated PIP(2) hydrolysis, Ca(2+) mobilization, arachidonic acid release, ERK1/2 and p38 MAP kinase phosphorylation, and stress fiber formation but inhibited cell proliferation. Human GPR54 was highly expressed in placenta, pituitary, pancreas, and spinal cord, suggesting a role in the regulation of endocrine function. Stimulation of oxytocin secretion after kisspeptin administration to rats confirmed this hypothesis.

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CRRD Object Information
CRRD ID: 1357971
Created: 2005-04-25
Species: All species
Last Modified: 2005-04-25
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.