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An antisense oligodeoxyribonucleotide to m-calpain mRNA inhibits myoblast fusion.

Authors: Balcerzak, D  Poussard, S  Brustis, J J  Elamrani, N  Soriano, M  Cottin, P  Ducastaing, A 
Citation: Balcerzak D, etal., J Cell Sci. 1995 May;108 ( Pt 5):2077-82.
Pubmed: (View Article at PubMed) PMID:7657725

Previous studies have led to the hypothesis of a possible role for m-calpain (EC in myoblast fusion in culture in vitro. To support this hypothesis, an antisense strategy has been used with cultured primary rat myoblasts. Using an appropriate antisense oligodeoxyribonucleotide to m-calpain mRNA, an inhibition of myoblast fusion has been observed, the maximum being obtained when the cell culture was treated with 30 microM of oligomer. Synthesis of m-calpain was decreased by 48% while high concentrations of antisense oligonucleotide do not significantly affect myoblast proliferation. The specificity of m-calpain intervention during fusion has also been confirmed using antisense oligonucleotides to mu-calpain and p94 mRNAs, respectively.


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CRRD Object Information
CRRD ID: 13792659
Created: 2018-09-19
Species: All species
Last Modified: 2018-09-19
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.