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MicroRNA-139-5p acts as a tumor suppressor by targeting ELTD1 and regulating cell cycle in glioblastoma multiforme.

Authors: Dai, Shouping  Wang, Xianjun  Li, Xiao  Cao, Yuandong 
Citation: Dai S, etal., Biochem Biophys Res Commun. 2015 Nov 13;467(2):204-10. doi: 10.1016/j.bbrc.2015.10.006. Epub 2015 Oct 9.
Pubmed: (View Article at PubMed) PMID:26449464
DOI: Full-text: DOI:10.1016/j.bbrc.2015.10.006

MicroRNA-139-5p was identified to be significantly down-regulated in glioblastoma multiform (GBM) by miRNA array. In this report we aimed to clarify its biological function, molecular mechanisms and direct target gene in GBM. Twelve patients with GBM were analyzed for the expression of miR-139-5p by quantitative RT-PCR. miR-139-5p overexpression was established by transfecting miR-139-5p-mimic into U87MG and T98G cells, and its effects on cell proliferation were studied using MTT assay and colony formation assays. We concluded that ectopic expression of miR-139-5p in GBM cell lines significantly suppressed cell proliferation and inducing apoptosis. Bioinformatics coupled with luciferase and western blot assays also revealed that miR-139-5p suppresses glioma cell proliferation by targeting ELTD1 and regulating cell cycle.


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CRRD Object Information
CRRD ID: 13838722
Created: 2019-01-09
Species: All species
Last Modified: 2019-01-09
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.