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Src suppressed C kinase substrate regulates the lipopolysaccharide-induced TNF-alpha biosynthesis in rat astrocytes.

Authors: Sun, Lin-lin  Cheng, Chun  Liu, Hai-ou  Shen, Cong-cong  Xiao, Feng  Qin, Jing  Yang, Jun-ling  Shen, Ai-guo 
Citation: Sun LL, etal., J Mol Neurosci. 2007;32(1):16-24.
Pubmed: (View Article at PubMed) PMID:17873284

The protein kinase C (PKC) is known to be a critical component in the signaling cascades that lead to astrocyte-activation. To further understand the mechanism of PKC signaling in astrocyte-activation, we investigated the effect of SSeCKS, a PKC substrate, on LPS-induced cytokine expression in astrocytes by RT-PCR and enzyme-linked immunosorbent assay. Exposure of the cells to LPS induced rapid translocation of SSeCKS to the perinuclear sides, ERK activation and pronounced TNF-alpha production, which can be inhibited by the PKC inhibitor Gö6983. By using siRNA knockdown of SSeCKS expression, LPS-induced signaling events were partly inhibited, including ERK activation, inducible TNF-alpha biosynthesis and secretion. These results suggest that SSeCKS is involved in the LPS-induced TNF-alpha expression in astrocytes mediated by PKC.


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CRRD Object Information
CRRD ID: 14348969
Created: 2019-02-05
Species: All species
Last Modified: 2019-02-05
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.