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Contribution of molecular analysis to the typification of the non-functioning pituitary adenomas.

Authors: Sanchez-Tejada, Laura  Sanchez-Ortiga, Ruth  Lamas, Cristina  Camara, Rosa  Riesgo, Pedro  Fajardo, Carmen  Aranda, Francisco Ignacio  Pico, Antonio 
Citation: Sanchez-Tejada L, etal., PLoS One. 2017 Jul 10;12(7):e0180039. doi: 10.1371/journal.pone.0180039. eCollection 2017.
Pubmed: (View Article at PubMed) PMID:28692683
DOI: Full-text: DOI:10.1371/journal.pone.0180039

AIM: The WHO Classification of Tumours of Endocrine Organs considers the inmunohistochemical characterization of pituitary adenomas (PA) as mandatory for patient diagnosis. Recent advances in the knowledge of the molecular patterns of these tumours could complement this classification with gene expression profiling.
METHODS: Within the context of the Spanish Molecular Registry of Pituitary Adenomas (REMAH), a multicentre clinical-basic research project, we analysed the molecular phenotype of 142 PAs with complete IHC and clinical information. Gene expression levels of all pituitary hormones, type 1 corticotrophin-releasing hormone receptor, dopamine receptors and arginine vasopressin receptor 1b were measured by quantitative real-time polymerase chain reaction. In addition, we used three housekeeping genes for normalization and a pool of nine healthy pituitary glands from autopsies as calibration reference standard.
RESULTS: Based on the clinically functioning PA (FPA: somatotroph, corticotroph, thyrotroph and lactotroph adenomas), we established the interquartile range of relative expression for all genes studied in each PA subtype. That allowed molecularly the different PA subtypes, including the clinically non-functioning PA (NFPA). Afterwards, we estimated the concordance of the molecular and immunohistochemical classification with clinical diagnosis in FPA and between them in NFPA. The kappa values were higher in molecular than in immunohistochemical classification in FPA and showed a bad concordance in all NFPA subtypes.
CONCLUSIONS: According to these results, the molecular characterization of the PA complements the IHC analysis, allowing a better typification of the NFPA.


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CRRD Object Information
CRRD ID: 14700668
Created: 2019-08-09
Species: All species
Last Modified: 2019-08-09
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.