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Efficient and rapid generation of large genomic variants in rats and mice using CRISMERE.

Authors: Birling, Marie-Christine  Schaeffer, Laurence  André, Philippe  Lindner, Loic  Maréchal, Damien  Ayadi, Abdel  Sorg, Tania  Pavlovic, Guillaume  Hérault, Yann 
Citation: Birling MC, etal., Sci Rep. 2017 Mar 7;7:43331. doi: 10.1038/srep43331.
Pubmed: (View Article at PubMed) PMID:28266534
DOI: Full-text: DOI:10.1038/srep43331

Modelling Down syndrome (DS) in mouse has been crucial for the understanding of the disease and the evaluation of therapeutic targets. Nevertheless, the modelling so far has been limited to the mouse and, even in this model, generating duplication of genomic regions has been labour intensive and time consuming. We developed the CRISpr MEdiated REarrangement (CRISMERE) strategy, which takes advantage of the CRISPR/Cas9 system, to generate most of the desired rearrangements from a single experiment at much lower expenses and in less than 9 months. Deletions, duplications, and inversions of genomic regions as large as 24.4 Mb in rat and mouse founders were observed and germ line transmission was confirmed for fragment as large as 3.6 Mb. Interestingly we have been able to recover duplicated regions from founders in which we only detected deletions. CRISMERE is even more powerful than anticipated it allows the scientific community to manipulate the rodent and probably other genomes in a fast and efficient manner which was not possible before.

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CRRD Object Information
CRRD ID: 14929209
Created: 2019-09-18
Species: All species
Last Modified: 2019-09-18
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.