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A novel desmocollin-2 mutation reveals insights into the molecular link between desmosomes and gap junctions.

Authors: Gehmlich, Katja  Lambiase, Pier D  Asimaki, Angeliki  Ciaccio, Edward J  Ehler, Elisabeth  Syrris, Petros  Saffitz, Jeffrey E  McKenna, William J 
Citation: Gehmlich K, etal., Heart Rhythm. 2011 May;8(5):711-8. doi: 10.1016/j.hrthm.2011.01.010. Epub 2011 Jan 7.
Pubmed: (View Article at PubMed) PMID:21220045
DOI: Full-text: DOI:10.1016/j.hrthm.2011.01.010


BACKGROUND: Cellular adhesion mediated by cardiac desmosomes is a prerequisite for proper electric propagation mediated by gap junctions in the myocardium. However, the molecular principles underlying this interdependence are not fully understood.
OBJECTIVE: The purpose of this study was to determine potential causes of right ventricular conduction abnormalities in a patient with borderline diagnosis of arrhythmogenic right ventricular cardiomyopathy.
METHODS: To assess molecular changes, the patient's myocardial tissue was analyzed for altered desmosomal and gap junction (connexin43) protein levels and localization. In vitro functional studies were performed to characterize the consequences of the desmosomal mutations.
RESULTS: Loss of plakoglobin signal was evident at the cell junctions despite expression of the protein at control levels. Although the distribution of connexin43 was not altered, total protein levels were reduced and changes in phosphorylation were observed. The truncation mutant in desmocollin-2a is deficient in binding plakoglobin. Moreover, the ability of desmocollin-2a to directly interact with connexin43 was abolished by the mutation. No pathogenic potential of the desmoglein-2 missense change was identified.
CONCLUSION: The observed abnormalities in gap junction protein expression and phosphorylation, which precede an overt cardiac phenotype, likely are responsible for slow myocardial conduction in this patient. At the molecular level, altered binding properties of the desmocollin-2a mutant may contribute to the changes in connexin43. In particular, the newly identified interaction between the desmocollin-2a isoform and connexin43 provides novel insights into the molecular link between desmosomes and gap junctions.

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CRRD Object Information
CRRD ID: 15014787
Created: 2019-11-09
Species: All species
Last Modified: 2019-11-09
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.