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Calcineurin functions in Ca(2+)-activated cell death in mammalian cells.

Authors: Shibasaki, F  McKeon, F 
Citation: Shibasaki F and McKeon F, J Cell Biol. 1995 Nov;131(3):735-43.
Pubmed: (View Article at PubMed) PMID:7593193

Calcineurin is a calcium-dependent protein phosphatase that functions in T cell activation. We present evidence that calcineurin functions more generally in calcium-triggered apoptosis in mammalian cells deprived of growth factors. Specifically, expression of epitope-tagged calcineurin A induces rapid cell death upon calcium signaling in the absence of growth factors. We show that this apoptosis does not require new protein synthesis and therefore calcineurin must operate through existing substrates. Co-expression of the Bcl-2 protooncogene efficiently blocks calcineurin-induced cell death. Significantly, we demonstrate that a calcium-independent calcineurin mutant induces apoptosis in the absence of calcium, and that this apoptotic response is a direct consequence of calcineurin's phosphatase activity. These data suggest that calcineurin plays an important role in mediating the upstream events in calcium-activated cell death.


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CRRD Object Information
CRRD ID: 1580726
Created: 2006-08-21
Species: All species
Last Modified: 2006-08-21
Status: ACTIVE


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