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Increased expression of PDGF A- and B-chain genes in rat lungs with hypoxic pulmonary hypertension.

Authors: Katayose, D  Ohe, M  Yamauchi, K  Ogata, M  Shirato, K  Fujita, H  Shibahara, S  Takishima, T 
Citation: Katayose D, etal., Am J Physiol. 1993 Feb;264(2 Pt 1):L100-6.
Pubmed: (View Article at PubMed) PMID:8447423

To study the molecular basis of vascular remodeling in pulmonary hypertension, we developed an experimental system in which male Sprague-Dawley rats were exposed to hypoxia for up to 3 wk. Both the right ventricular systolic pressure and gravimetric index for right ventricular hypertrophy were higher in rats exposed to hypoxia for 3 wk than those of age-matched control rats (P < 0.01), indicating that pulmonary hypertension was established under conditions used. To examine the possible involvement of platelet-derived growth factor (PDGF) in the pulmonary vascular remodeling caused by hypoxia, we cloned rat PDGF A- and B-chain cDNA and prepared specific cRNA probes. Northern blot analysis revealed that PDGF B-chain mRNA levels in the lungs were increased, reached a maximum of day 1, and were sustained at day 3, whereas PDGF A-chain mRNA levels reached a maximum on day 3. Thus the increase in the PDGF B-chain mRNA level precedes that in the PDGF A-chain mRNA level. These results suggest that the PDGF A- and B-chain products may be coordinately and sequentially involved in hypoxic pulmonary vascular remodeling.


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CRRD Object Information
CRRD ID: 1580847
Created: 2006-08-29
Species: All species
Last Modified: 2006-08-29
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.