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Pituitary synaptic protein SNAP-25 sensitive to GnRH is necessary for LH release.

Authors: Quintanar, JL  Salinas, E  Chavez-Morales, RM  Quintanar-Stephano, A 
Citation: Quintanar JL, etal., Endocr Regul. 2004 Mar;38(1):1-6.
Pubmed: (View Article at PubMed) PMID:15147232

OBJECTIVE: The protein SNAP-25 is located in the plasma membrane and is known to participate in hormone exocytosis process. In the present work we studied the role of SNAP-25 on LH secretion in permeabilized adenohypophyseal cultured cells. The question of whether GnRH regulates SNAP-25 expression in adenohypophyseal cultured cells and in the adenohypophyses in vivo was also investigated. METHODS: In digitonin-permeabilized cells incubated with anti-SNAP-25, stimulated LH secretion with Ca2+ was analysed. The presence and expression of SNAP-25 in adenohypophyseal cultured cells incubated with GnRH and in adenohypophyses of orchidectomized rats with GnRH administration was studied by immunochemistry and immunoblotting. RESULTS: Immunochemical study revealed that SNAP-25 was present in cultured adenohypophyseal cells and in adenohypophysis of orchidectomized rats both with GnRH treatment. We found that LH secretion can be blocked by antibodies raised against SNAP-25 in permeabilized cells. Likewise, GnRH administration induced a significant decrease of SNAP-25 expression in cultured adenohypophyseal cells and in adenohypophysis of orchidectomized rats. CONCLUSION: Our study showed that SNAP-25 is present in adenohypophyses in vitro as well as in vivo and that is involved in LH release and that GnRH can modify its expression.


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CRRD Object Information
CRRD ID: 1581080
Created: 2006-09-14
Species: All species
Last Modified: 2006-09-14
Status: ACTIVE


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