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Differences in matrix metalloproteinase-1 and matrix metalloproteinase-12 transcript levels among carotid atherosclerotic plaques with different histopathological characteristics.

Authors: Morgan, AR  Rerkasem, K  Gallagher, PJ  Zhang, B  Morris, GE  Calder, PC  Grimble, RF  Eriksson, P  McPheat, WL  Shearman, CP  Ye, S 
Citation: Morgan AR, etal., Stroke. 2004 Jun;35(6):1310-5. Epub 2004 Apr 8.
Pubmed: (View Article at PubMed) PMID:15073384
DOI: Full-text: DOI:10.1161/01.STR.0000126822.01756.99

BACKGROUND AND PURPOSE: Previous studies have shown that atherosclerotic lesions express a number of matrix metalloproteinases (MMPs). Here we investigated whether transcript levels of MMP-1, -3, -7, -9, and -12 in carotid atherosclerotic plaques were correlated with histological features and clinical manifestations. METHODS: Atherosclerotic plaques (n=50) removed from patients undergoing carotid endarterectomy were classified histologically using a system proposed by Virmani et al, and MMP-1, -3, -7, -9, and -12 transcript levels in these tissues were quantified by real-time reverse-transcriptase polymerase chain reaction. RESULTS: Compared to plaques with a thick fibrous cap, those with a thin cap had a 7.8-fold higher MMP-1 transcript level (P=0.006). MMP-3, -7, and -12 were 1.5-fold, 1.8-fold, and 2.1-fold, respectively, higher in thin cap plaques, but the differences did not reach statistical significance. MMP-12 transcript levels were significantly increased in ruptured plaques compared with lesions without cap disruption (P=0.001). MMP-9 transcript levels were similar among the different types of lesion. MMP-1 and -12 transcript levels were significantly higher in plaques from patients with amaurosis fugax, than in those from asymptomatic patients (P=0.029 and P=0.008 for MMP-1 and MMP-12, respectively), than in those from patients with stroke (P=0.027 and P=0.001, respectively), and than in those from patients with transient ischemic attack (P=0.046 and P=0.008, respectively). CONCLUSIONS: These data support a role of MMP-1 and -12 in determining atherosclerotic plaque stability.


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CRRD Object Information
CRRD ID: 1582361
Created: 2006-11-06
Species: All species
Last Modified: 2006-11-06
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.