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Cellular localization and interaction of ABCA1 and caveolin-1 in aortic endothelial cells after HDL incubation.

Authors: Chao, WT  Tsai, SH  Lin, YC  Lin, WW  Yang, VC 
Citation: Chao WT, etal., Biochem Biophys Res Commun. 2005 Jul 8;332(3):743-9.
Pubmed: (View Article at PubMed) PMID:15907796
DOI: Full-text: DOI:10.1016/j.bbrc.2005.05.019

The goal of this study was to investigate the cellular localization and the interaction between caveolin-1 and ABCA1 in cholesterol-loaded aortic endothelial cells after HDL incubation. Immunofluorescence confocal microscopy showed that ABCA1 was found primarily on the cell surface, whereas caveolin-1 was revealed on the cell surface and in the cytoplasm. The HDL appeared to colocalize with ABCA1 and caveolin-1 on the cell surface. No free HDL was revealed in the cytoplasm. The HDL was colocalized neither with early endosome marker (CD71) nor with late endosome marker (LAMP2). The chemical cross-linking and immunoprecipitation analysis revealed that ABCA1 binds directly to both HDL and caveolin-1, whereas HDL does not bind directly to caveolin-1. The studies provide evidence for a direct interaction between ABCA1 and HDL, ABCA1 and caveolin-1, but not HDL and caveolin-1, indicating that ABCA1 may act as a structural platform between HDL and caveolin-1 on the cell surface during cellular cholesterol efflux.


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CRRD Object Information
CRRD ID: 1598535
Created: 2006-12-02
Species: All species
Last Modified: 2006-12-02
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.