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Requirement of GM2 ganglioside activator for phospholipase D activation.

Authors: Nakamura, S  Akisue, T  Jinnai, H  Hitomi, T  Sarkar, S  Miwa, N  Okada, T  Yoshida, K  Kuroda, S  Kikkawa, U  Nishizuka, Y 
Citation: Nakamura S, etal., Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12249-53.
Pubmed: (View Article at PubMed) PMID:9770472

Sequence analysis of a heat-stable protein necessary for the activation of ADP ribosylation factor-dependent phospholipase D (PLD) reveals that this protein has a structure highly homologous to the previously known GM2 ganglioside activator whose deficiency results in the AB-variant of GM2 gangliosidosis. The heat-stable activator protein indeed has the capacity to enhance enzymatic conversion of GM2 to GM3 ganglioside that is catalyzed by beta-hexosaminidase A. Inversely, GM2 ganglioside activator purified separately from tissues as described earlier [Conzelmann, E. & Sandhoff, K. (1987) Methods Enzymol. 138, 792-815] stimulates ADP ribosylation factor-dependent PLD in a dose-dependent manner. At higher concentrations of ammonium sulfate, the PLD activator protein apparently substitutes for protein kinase C and phosphatidylinositol 4,5-bisphosphate, both of which are known as effective stimulators of the PLD reaction. The mechanism of action of the heat-stable PLD activator protein remains unknown.

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CRRD Object Information
CRRD ID: 1598995
Created: 2007-01-10
Species: All species
Last Modified: 2007-01-10
Status: ACTIVE



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