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Purification of adenine phosphoribosyltransferase by affinity chromatography.

Authors: Hershey, HV  Taylor, MW 
Citation: Hershey HV and Taylor MW, Prep Biochem. 1978;8(6):453-62.
Pubmed: (View Article at PubMed) PMID:748918
DOI: Full-text: DOI:10.1080/00327487808061662

The purine salvage pathway enzyme adenine phosphoribosyltransferase (AMP: pyrophosphate phosphoribosyltransferase EC 2.42.7) has been purified to greater than 85% homogeneity from crude rat liver 100,000 x g supernatant in one step by affinity chromatography. The enzyme binds to an AMP-agarose column and is eluted off the column by 1 mM 5-phosphoribosyl pyrophosphate with a 50 to 80% recovery. Enzyme kinetics indicate that the mechanism of the specific elution is due to competition of the product AMP and substrate 5-phosphoribosyl pyrophosphate for the same site on the enzyme.


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CRRD Object Information
CRRD ID: 1599205
Created: 2007-01-19
Species: All species
Last Modified: 2007-01-19
Status: ACTIVE


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