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Ca2+ uptake coupled to glycogen phosphorolysis in the glycogenolytic-sarcoplasmic reticulum complex from rat skeletal muscle.

Authors: Nogues, M  Cuenda, A  Henao, F  GutiĆ©rrez-Merino, C 
Citation: Nogues M, etal., Z Naturforsch C. 1996 Jul-Aug;51(7-8):591-8.
Pubmed: (View Article at PubMed) PMID:8810099

The glycogenolytic-sarcoplasmic reticulum complex from rat skeletal muscle accumulates Ca2+ upon stimulation of glycogen phosphorolysis in the absence of added ATP. It is shown that an efficient Ca2+ uptake involves the sequential action of glycogen phosphorylase, phosphoglucomutase and hexokinase, which generate low concentrations of ATP (approximately 1-2 microM) compartmentalized in the immediate vicinity of the sarcoplasmic reticulum Ca2+, Mg(2+)-ATPase (the Ca2+ pump). The Ca2+ uptake supported by glycogenolysis in this subcellular structure is strongly stimulated by micromolar concentrations of AMP, showing that the glycogen phosphorylase associated with this complex is in the dephosphorylated b form. The results point out that the flux through this compartmentalized metabolic pathway should be enhanced in physiological conditions leading to increased AMP concentrations in the sarcoplasm, such as long-lasting contractions and in ischemic muscle.

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CRRD Object Information
CRRD ID: 1599992
Created: 2007-02-22
Species: All species
Last Modified: 2017-01-03
Status: ACTIVE



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