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Evidence for cyclooxygenase-2 association with caveolin-3 in primary cultured rat chondrocytes.

Authors: Kwak, JO  Lee, WK  Kim, HW  Jung, SM  Oh, KJ  Jung, SY  Huh, YH  Cha, SH 
Citation: Kwak JO, etal., J Korean Med Sci. 2006 Feb;21(1):100-6.
Pubmed: (View Article at PubMed) PMID:16479074
DOI: Full-text: DOI:10.3346/jkms.2006.21.1.100

The purpose of this study was to demonstrate the cellular localization of cyclooxygenase-2 (COX-2) and caveolin-3 (Cav-3) in primarily cultured rat chondrocytes. In normal rat chondrocytes, we observed relatively high levels of Cav-3 and a very low level of COX-2 mRNA and protein. Upon treating the chondrocytes with 5 microM of CdCl(2) (Cd) for 6 hr, the expressions of COX-2 mRNA and protein were increased with the decreased Cav-3 mRNA and protein expressions. The detergent insoluble caveolae-rich membranous fractions that were isolated from the rat chondrocytes and treated with Cd contained the both proteins of both COX-2 and Cav-3 in a same fraction. The immuno-precipitation experiments showed complex formation between the COX-2 and Cav-3 in the rat chondrocytes. Purified COX-2 with glutathione S-transferase-fused COX-2 also showed complex formation with Cav-3. Confocal and electron microscopy also demonstrated the co-localization of COX-2 and Cav-3 in the plasma membrane. The results from our current study show that COX-2 and Cav-3 are co-localized in the caveolae of the plasma membrane, and they form a protein-protein complex. The co-localization of COX-2 with Cav-3 in the caveolae suggests that the caveolins might play an important role for regulating the function of COX-2.


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CRRD Object Information
CRRD ID: 1600265
Created: 2007-03-06
Species: All species
Last Modified: 2007-03-06
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.