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Effect of N-ethylmaleimide on beef and rat liver vitamin K1 epoxide reductase.

Authors: Silverman, RB  Nandi, DL 
Citation: Silverman RB and Nandi DL, J Enzyme Inhib. 1990;3(4):289-94.
Pubmed: (View Article at PubMed) PMID:2319331

There is little difference in the extent of inactivation of beef liver microsomal vitamin K1 epoxide reductase by N-ethylmaleimide (NEM) whether or not the microsomes are pre-treated with dithiothreitol (DTT). The rat liver microsomal enzyme, however, is inactivated by NEM to a much greater extent if the microsomes are pre-treated with DTT. The beef liver enzyme activity is protected from NEM inactivation by the substrate, vitamin K1 epoxide. Ping-pong kinetics are exhibited by the beef liver enzyme. These results support a mechanism for vitamin K1 epoxide reductase in which the function of the required dithiol is to reduce an active site disulfide bond; however, the geometry of the active sites of the enzyme from rat and beef may be different.


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CRRD Object Information
CRRD ID: 1600461
Created: 2007-03-09
Species: All species
Last Modified: 2007-03-09
Status: ACTIVE


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