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Expression of Tie-2 and angiopoietin-1 and -2 in early phase of ulcer healing.

Authors: Wen, CY  Ito, M  Chen, LD  Matsuu, M  Shichijo, K  Nakayama, T  Nakashima, M  Xu, ZM  Ohtsuru, A  Hsu, CT  Sekine, I 
Citation: Wen CY, etal., J Gastroenterol. 2003;38(5):431-5.
Pubmed: (View Article at PubMed) PMID:12768384
DOI: Full-text: DOI:10.1007/s00535-002-1078-3

BACKGROUND: Angiogenesis is an important process in tissue development and wound healing. The Tie-2 receptor tyrosine kinases and ligands, angiopoietin (Ang)-1 and -2 have been postulated to play key roles in vascular development. The purpose of this study was to evaluate the expression of Tie-2 and Ang-1 and -2 in an acetic acid-induced gastric ulcer healing process in rats. METHODS: Gastric specimens were obtained at 0 (control), 1, 3, 5, 7, and 14 days after ulcer induction for reverse transcription polymerase chain reaction (RT-PCR), Western blot analysis, and immunohistochemical analysis. RESULTS: Expression of Tie-2 and Ang-1 and -2 mRNAs was detected in normal gastric tissue and ulcerative tissues by RT-PCR. Western blot analysis revealed that Tie-2 expression reached a maximum on the third to fifth days. Expression of Ang-1 and -2 peaked on the first day. Ang-1 expression gradually became weaker in 2 weeks, whereas Ang-2 expression returned to normal in a few days. Immunohistochemically, Tie-2 was expressed constitutively in the endothelial cells of pre-existing vessels of the gastric wall, and Tie-2 expression was increased in the new capillaries of the ulcer base. CONCLUSIONS: These findings suggest that Tie-2 and Ang-1 and -2 play an important role in angiogenesis in the early phase of ulcer healing.

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CRRD Object Information
CRRD ID: 1601496
Created: 2007-04-23
Species: All species
Last Modified: 2007-04-23
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.