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The serum protein alpha2-HS glycoprotein/fetuin inhibits apatite formation in vitro and in mineralizing calvaria cells. A possible role in mineralization and calcium homeostasis.

Authors: Schinke, T  Amendt, C  Trindl, A  Poschke, O  Muller-Esterl, W  Jahnen-Dechent, W 
Citation: Schinke T, etal., J Biol Chem. 1996 Aug 23;271(34):20789-96.
Pubmed: (View Article at PubMed) PMID:8702833

We present data suggesting a function of alpha2-HS glycoproteins/fetuins in serum and in mineralization, namely interference with calcium salt precipitation. Fetuins occur in high serum concentration during fetal life. They accumulate in bones and teeth as a major fraction of noncollagenous bone proteins. The expression pattern in fetal mice confirms that fetuin is predominantly made in the liver and is accumulated in the mineralized matrix of bones. We arrived at a hypothesis on the molecular basis of fetuin function in bones using primary rat calvaria osteoblast cultures and salt precipitation assays. Our results indicate that fetuins inhibit apatite formation both in cell culture and in the test tube. This inhibitory effect is mediated by acidic amino acids clustering in cystatin-like domain D1. Fetuins account for roughly half of the capacity of serum to inhibit salt precipitation. We propose that fetuins inhibit phase separation in serum and modulate apatite formation during mineralization.


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CRRD Object Information
CRRD ID: 1626074
Created: 2007-07-09
Species: All species
Last Modified: 2007-07-09
Status: ACTIVE


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