Combinations of TLR and NOD2 ligands stimulate rat microglial P2X4R expression.

Authors: Guo, LH  Guo, KT  Wendel, HP  Schluesener, HJ 
Citation: Guo LH, etal., Biochem Biophys Res Commun. 2006 Oct 27;349(3):1156-62. Epub 2006 Sep 1.
Pubmed: (View Article at PubMed) PMID:16973131
DOI: Full-text: DOI:10.1016/j.bbrc.2006.08.146

As ATP-gated ion channels, P2X4 receptors (P2X4R) of microglial cells play a crucial role in central nervous system (CNS) inflammation. In this study, we used rat microglial cell cultures to examine P2X4R expression in response to stimulation by combination of toll-like receptors (TLRs) and nucleotide-binding oligomerization domain 2 (NOD2) receptors. Various TLR1-9 ligands and NOD2 agonist muramyldipeptide (MDP) were investigated. Our results showed that certain combination of ligands had additive effects on upregulating microglial P2X4R at both mRNA and protein levels, and induced nitric oxide increase and tumor necrosis factor-alpha production. Thus TLRs and NOD2 combinations are contributors to the signaling cascades resulting in purinergic microglial activation.

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CRRD Object Information
CRRD ID: 1643119
Created: 2007-11-30
Species: All species
Last Modified: 2007-11-30
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.