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Epigenetic silenced miR-125a-5p could be self-activated through targeting Suv39H1 in gastric cancer.

Authors: Cai, Mingzhi  Chen, Qiuxian  Shen, Juntao  Lv, Chenbing  Cai, Lisheng 
Citation: Cai M, etal., J Cell Mol Med. 2018 Oct;22(10):4721-4731. doi: 10.1111/jcmm.13716. Epub 2018 Aug 17.
Pubmed: (View Article at PubMed) PMID:30117667
DOI: Full-text: DOI:10.1111/jcmm.13716

Emerging evidence suggests that microRNAs (miRNAs) serve an important role in tumorigenesis and development. Although the low expression of miR-125a-5p in gastric cancer has been reported, the underlying mechanism remains unknown. In the current study, the low expression of miR-125a-5p in gastric cancer was verified in paired cancer tissues and adjacent non-tumour tissues. Furthermore, the GC islands in the miR-125a-5p region were hypermethylated in the tumour tissues. And the hypermethylation was negatively correlated with the miR-125a-5p expression. Target gene screening showed that the histone methyltransferase Suv39H1 was one of the potential target genes. In vitro studies showed that miR-125a-5p could directly suppress the Suv39H1 expression and decrease the H3K9me3 levels. On the other hand, the Suv39H1 could induce demethylation of miR-125a-5p, resulting in re-activation of miR-125a-5p. What is more, overexpessing miR-125a-5p could also self-activate the silenced miR-125a-5p in gastric cancer cells, which suppressed cell migration, invasion and proliferation in vitro and inhibited cancer progression in vivo. Thus, we uncovered here that the epigenetic silenced miR-125a-5p could be self-activated through targeting Suv39H1 in gastric cancer, suggesting that miR-125a-5p might be not only the potential prognostic value as a tumour biomarker but also potential therapeutic targets in gastric cancer.

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CRRD ID: 21403678
Created: 2020-03-09
Species: All species
Last Modified: 2020-03-09
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.