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Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance.

Authors: Hashiguchi, Yojiro  Nishida, Naohiro  Mimori, Koshi  Sudo, Tomoya  Tanaka, Fumiaki  Shibata, Kohei  Ishii, Hideshi  Mochizuki, Hidetaka  Hase, Kazuo  Doki, Yuichiro  Mori, Masaki 
Citation: Hashiguchi Y, etal., Int J Oncol. 2012 May;40(5):1477-82. doi: 10.3892/ijo.2012.1363. Epub 2012 Feb 9.
Pubmed: (View Article at PubMed) PMID:22322911
DOI: Full-text: DOI:10.3892/ijo.2012.1363

Recent reports have demonstrated that another strand of mature microRNA (miRNA), called microRNA* or 3p (5p) strand, which is generated from the same precursor miRNA (Pre-miR), has a crucial role in cellular function. We previously reported the tumor suppressive effect of miR-125a-5p in gastric cancer. The current study was designed to examine the function and clinical significance of miR-125a-3p, a partner strand of miR-125a-5p, in human gastric cancer. Quantitative RT-PCR was used to evaluate miR-125a-3p expression in 70 gastric cancer cases to determine the clinicopathologic significance of miR-125a-3p expression. In addition, the effect of miR-125a-3p on the proliferation of gastric cancer cells was investigated. Low expression levels of miR-125a-3p were associated with indicators of enhanced malignant potential such as tumor size (p=0.0002), tumor invasion (p=0.0149), lymph node metastasis (p=0.018), liver metastasis (p=0.016), peritoneal dissemination (p=0.03), advanced clinical stage (p=0.0037) and poor prognosis (p=0.0083). Multivariate analysis indicated that low miR-125a-3p expression was an independent prognostic factor for survival, while in vitro assays demonstrated that miR‑125a-3p suppressed the proliferation of gastric cancer cells. MiR-125a-3p is a potent prognostic marker in gastric cancer. The clinical significance and tumor suppressive effect of miR‑125a-3p, as well as previously reported miR-125a-5p, suggest that the functional role of another strand of the mature form miRNA cannot be ignored, at least in miR-125a biogenesis.

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CRRD ID: 21408544
Created: 2020-03-12
Species: All species
Last Modified: 2020-03-12
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.