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Generation of rat mutants using a coat color-tagged Sleeping Beauty transposon system.

Authors: Lu, B  Geurts, AM  Poirier, C  Petit, DC  Harrison, W  Overbeek, PA  Bishop, CE 
Citation: Lu B, etal., Mamm Genome. 2007 May;18(5):338-46. Epub 2007 Jun 8.
Pubmed: (View Article at PubMed) PMID:17557177
DOI: Full-text: DOI:10.1007/s00335-007-9025-5

A significant barrier to exploiting the full potential of the rat as a biomedical model is the lack of tools to easily modify its germline. Here we show that a tyrosinase-tagged Sleeping Beauty transposon can be used as a simple, efficient method to generate rat mutants in vivo. By making two lines of transgenic rats, one carrying the transposon and another expressing the transposase in germ cells, we are able to obtain bigenic males in which transposition occurs in the germ cells. We show that transposition leads to the appearance of new coat colors in the offspring. Using such bigenic males, we obtained an average of 1.2 transpositions per gamete and identified 19 intragenic integration events among 96 transposition sites that were sequenced. In addition, gene trapping was confirmed and rats with evidence for transposon-induced dominant ocular anomalies were identified. These data suggest that the modified Sleeping Beauty transposon represents a powerful new tool for producing molecularly defined mutagenesis in the rat.


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CRRD Object Information
CRRD ID: 2290040
Created: 2008-02-25
Species: All species
Last Modified: 2008-02-25
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.