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[Changes of matrix metalloproteinase 2,9 and tissue inhibitor of metalloproteinase 1, 2, 3 expression level in aged rat lung.]

Authors: Zhao, ZJ  Liu, XM  Zhou, GP  Bu, DF  Li, XY  Zong, LL  Li, J 
Citation: Zhao ZJ, etal., Beijing Da Xue Xue Bao. 2008 Feb 18;40(1):101-104.
Pubmed: (View Article at PubMed) PMID:18278151

OBJECTIVEs:To investigate the underlying mechanism of age related increase of pulmonary fibrosis incidenceh METHODS: The levels of pulmonary collagen and TGF-&bgr;1 protein, MMP2,9 mRNA and TIMP 1,2,3 mRNA expression were measured by ELISA and realtime PCR. RESULTS: As compared with adult rats [(756±160) pg/g vs (1 000±246) pg/g, P<0.05] the aged rats had more extracellular matrix components, increased TGF-beta1 protein level, lower (3.15±1.76 vs 0.17±0.13, P<0.01) MMP2 mRNA expression and TIMP 1,2,3 mRNA expressions (TIMP1 2.00±1.74 vs 0.11± 0.06,TIMP2 7.60±2.51 vs 2.69±1.76, TIMP3 1.32±0.46 vs 0.29±0.16, P<0.01). No difference was observed in MMP9 (1.66±0.67 vs 1.74±0.87, P>0.05) mRNA expression. CONCLUSION: elevation in TGF-beta1 protein level, changes in the expression of MMP2/9 and decreased expression of TIMPS mRNA could be the underlying mechanism of age related increase of pulmonary incidence.

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CRRD Object Information
CRRD ID: 2290357
Created: 2008-03-10
Species: All species
Last Modified: 2008-03-10
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.