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Effect of Ca(2+) and Mg(2+) on the Mn-superoxide dismutase from rat liver and heart mitochondria.

Authors: Perez-Vazquez, V  Ramirez, J  Aguilera-Aguirre, L  Gonzalez-Hernandez, JC  Clemente-Guerrero, M  Manzo-Avalos, S  Uribe, S  Saavedra-Molina, A 
Citation: Perez-Vazquez V, etal., Amino Acids. 2002 Jun;22(4):405-16.
Pubmed: (View Article at PubMed) PMID:12107766
DOI: Full-text: DOI:10.1007/s007260200024

The manganese superoxide dismutase (Mn-SOD) converts superoxide anions to hydrogen peroxide plus oxygen, providing the first line of defense against oxidative stress in mitochondria. Heart mitochondria exhibited higher Mn-SOD activity than liver mitochondria. In mitochondria from both tissues Mn-SOD activity decreased after incubation at low oxygen concentration (hypoxic mitochondria). The effects of free Ca(2+) ([Ca(2+)](f)) and free Mg(2+) ([Mg(2+)](f)) on normoxic and hypoxic mitochondria from either organ were tested. In normoxic mitochondria from either tissue, both [Ca(2+)](f) and [Mg(2+)](f) activated the enzyme, although [Mg(2+)](f) was less efficient as an activator and the effect was lower in heart than in liver mitochondria. When added simultaneously, high [Ca(2+)](f) and [Mg(2+)](f) exhibited additive effects which were more pronounced in heart mitochondria and were observed regardless of whether mitochondria had been incubated under normal or low oxygen. The data suggest that [Ca(2+)](f) plays a role in regulating Mn-SOD in concert with the activation of aerobic metabolism.

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CRRD Object Information
CRRD ID: 2291939
Created: 2008-04-04
Species: All species
Last Modified: 2008-04-04
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.